Deborah E. Lycan
Professor of Biology and Biochemistry/Molecular Biology
Bio 200 Investigations in Cell and Molecular Biology
Bio 311 Molecular Biology
Bio 312 Recombinant DNA Lab
BCMB 410 Biochemistry/Molecular Biology Seminar
Bio 320 Human Genes and Disease
Current research interests and projects
Our research focuses on how ribosomal subunits are assembled in eukaryotic cells. We use the yeast S. cerevisiae as a model organism to study this essential and highly conserved process. Ribosomes are among the largest and most complex macromolecular machines assembled in cells. Ribosomes are assembled from imported ribosomal proteins on nascent rRNA in the nucleolus of the cell. The large and small subunits are then independently exported through nuclear pores at rates that can reach 30 subunits per second. Both subunits require the export receptor Crm1 for export, and both undergo independent post-export maturation events that are required before the subunits are translation-ready. Over 150 proteins that do not end up as constituents of the mature ribosome are necessary for ribosome biogenesis. The molecular details of how most of these non-ribosomal proteins function is unknown.
We have focused our work on the late steps of small subunit biogenesis. In particular, we are characterizing the role of Ltv1, a late adding biogenesis factor, and the role of RpS3, a ribosomal protein that interacts with Ltv1. We hope to understand better what role ribosomal proteins play in ribosome biogenesis and what role Ltv1 plays in both export and subunit maturation.
Post-doctoral Fellow, Harvard Medical School, PhD University of Colorado, Boulder, BA University of California, San Diego