BCMB Seminar: Simran Handa ’19
Date: 4:00pm PDT April 17 Location: JR Howard 116
JR Howard 116
Please join us for the BCMB seminar this Wednesday. Simran Handa ’19 will be presenting her research.
Date/time: April 17 at 4 pm (come a little early for snacks!)
Place: J. R. Howard 116
Title: Investigating the putative GEF of a Rab GTPase in lysosome-related organelle biogenesis
Abstract: Rab GTPases (Rabs) are proteins that are spatial and temporal regulators of organelle dynamics within cells. Rabs function in intracellular transport and organelle biogenesis as molecular switches, having two main conformations: a GTP-bound active form that can bind effectors, and a GDP-bound inactive form that cannot. Guanine-exchange factors (GEFs) activate Rabs by catalyzing GDP to GTP exchange. I present genetic and biochemical data proposing a new GEF for GLO-1, a Rab GTPase involved in lysosome-related organelle biogenesis in C. elegans. I found that the two subunits of the putative GEF complex—GLO-3 and CCZ-1—physically interact with each other and inactive GLO-1(GDP), pointing toward their role as a GEF.
Related Paper: https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007772