Protocols

Many of the protocols we use in lab will be available here for download soon.  Keep checking back for updates!

Bioinformatics

Clustering sequences with mothur (.doc)

 

DNA extraction

REDE extraction: tips & tissue (.doc)
Qiagen DNeasy extraction protocol for Frankia (.doc)

 

Enzyme Activity Assays

Enzymatic activity assay for ectomycorrhizal root tips (.doc)

 

Media, Solutions, and More

Agarose (.doc)
Blue juice (.doc)
DNA ladder (.doc)
Modified Melin-Norkrans (MMN) (.doc)

 

PCR, RFLP, Sequencing, and More

nif H PCR (for Frankia) (.doc)

ITS PCR (for fungi) (.doc)

Gel electrophoresis (.doc)
RFLP digests (.doc)
DNA sequencing (including PCR clean-up) (.doc)

Seed germination 

Arbutus menziesii (Pacific madrone) (.doc)